Research Articles
Overcoming Metabolic Burden in Engineered Microbial Strains: Strategies for Robust Bioproduction
Metabolic burden, the physiological stress imposed on cells by engineered pathways, remains a significant bottleneck in developing efficient microbial cell factories for therapeutic and high-value compound production.
Advanced Screening Methods for Amino Acid Overproducers: From Foundational Concepts to Biomedical Applications
This article provides a comprehensive analysis of modern screening strategies for amino acid overproducing strains, which are crucial for advancing microbial fermentation in biomedical and pharmaceutical industries.
Metabolic Engineering for Renewable Resource Utilization: From Foundational Principles to Advanced Applications
This article provides a comprehensive overview of metabolic engineering strategies for the efficient utilization of renewable resources, with a focus on lignocellulosic biomass.
13C Metabolic Flux Analysis: A Comprehensive Guide from Foundational Principles to Advanced Applications in Biomedical Research
This article provides a comprehensive guide to 13C Metabolic Flux Analysis (13C-MFA), a cornerstone technique for quantifying intracellular metabolic fluxes in living cells.
Modular Metabolic Engineering: A Strategic Framework for Optimizing Biobased Chemical Production
This article provides a comprehensive overview of modular metabolic engineering (MME), a transformative approach that addresses the challenges of metabolic imbalance in engineered microbial strains.
Engineering Novel Metabolism: A Systems Biology Guide to Non-Native Reaction Insertion
The insertion of non-native reactions into metabolic networks is a cornerstone of synthetic biology, enabling the production of high-value chemicals and advanced therapeutics.
Engineering Isoprenoid Biosynthesis: Pathways, Progress, and Pharmaceutical Applications
This article provides a comprehensive overview of the latest advances in isoprenoid biosynthetic pathway engineering, a field critical for the sustainable production of valuable therapeutics, nutraceuticals, and biofuels.
Pooled CRISPR Screening for Strain Tolerance: A Comprehensive Guide from Screen Design to Hit Validation
Pooled CRISPR screening has emerged as a powerful, high-throughput methodology for unbiased discovery of genetic determinants of strain tolerance, with profound implications for bioproduction, drug discovery, and functional genomics.
Metabolic Engineering for Fatty Acid-Derived Biofuels: Advanced Strategies in Yeast and Microbial Hosts
This article comprehensively reviews the current landscape of metabolic engineering for the production of high-energy-density, fatty acid-derived biofuels.
Advanced Biosensor Development for High-Throughput Metabolite Screening: Applications in Metabolic Engineering and Drug Discovery
This article provides a comprehensive overview of the latest advancements in biosensor technology for high-throughput metabolite screening, catering to researchers, scientists, and drug development professionals.