Research Articles
CRISPRi Metabolic Engineering: Rewiring E. coli Feedback Loops for High-Yield Bioproduction
This article provides a comprehensive guide for researchers on applying CRISPR interference (CRISPRi) to manipulate feedback inhibition in Escherichia coli metabolic pathways.
Engineering Tomorrow's Biomanufacturing: A Guide to CRISPR Genome Editing for Microbial Cell Factories
This comprehensive guide explores CRISPR-based genome editing for engineering microbial cell factories, tailored for researchers and bioprocess professionals.
CRISPR-Driven Modular Metabolic Engineering: Designing Cellular Factories for Precision Therapeutics and Biomanufacturing
This article provides a comprehensive guide for researchers and industry professionals on implementing CRISPR-Cas systems for modular metabolic engineering (MME).
CRISPR Efficiency Showdown: Why Pichia Pastoris Outperforms E. coli and S. cerevisiae for Protein Expression
This article provides a comprehensive comparative analysis of CRISPR-Cas editing efficiency across major microbial hosts, with a focused evaluation of the methylotrophic yeast Pichia pastoris (Komagataella phaffii).
Unlocking Cellular Resilience: A Comprehensive Guide to CRISPRa Screens for Tolerance Phenotypes
This article provides a comprehensive guide for researchers on leveraging CRISPR activation (CRISPRa) screens to systematically discover genes that confer tolerance to cellular stress, therapeutic agents, or disease conditions.
Engineering Green Factories: A CRISPR-Cas9 Guide to Isoprenoid Production in Microalgae
This article provides a comprehensive technical guide for researchers and industry professionals on leveraging CRISPR-Cas9 for the metabolic engineering of microalgae to produce high-value isoprenoids.
CRISPR-Cas9 Biofuel Pathway Engineering: A Comprehensive Guide for Metabolic Engineers and Biotechnologists
This article provides a detailed exploration of CRISPR-Cas9 genome editing for engineering microbial and plant systems to enhance biofuel production.
A Comprehensive CRISPR-Cas9 Protocol for Pichia pastoris: From Design to Validation for Drug Development
This article provides a detailed, step-by-step protocol for implementing CRISPR-Cas9 gene editing in the methylotrophic yeast Pichia pastoris (Komagataella phaffii), a critical host for recombinant protein and therapeutic drug production.
CRISPR-Cas Genome Engineering for Chassis Development: A Comprehensive Guide for Synthetic Biology and Drug Discovery
This article provides a detailed, current guide for researchers and drug development professionals on leveraging CRISPR-Cas systems for advanced chassis organism engineering.
COBRA Toolbox: The Essential Guide to Metabolic Flux Analysis for Biomedical Research and Drug Discovery
This comprehensive guide explores the COnstraint-Based Reconstruction and Analysis (COBRA) Toolbox, the leading open-source platform for genome-scale metabolic modeling and flux analysis.