Research Articles
Alternate Optimal Solutions in Flux Balance Analysis: A Comprehensive Guide for Metabolic Modeling and Drug Discovery
This article provides a comprehensive guide to the theory, identification, and application of alternate optimal solutions (AOS) in Flux Balance Analysis (FBA) for researchers and drug development professionals.
Optimizing Succinic Acid Bioproduction: A Cuckoo Search Algorithm Approach for Enhanced Microbial Fermentation
This article explores the application of the nature-inspired Cuckoo Search (CS) optimization algorithm to enhance the bioproduction of succinic acid, a key platform chemical in pharmaceuticals and biomedicine.
Kinetic vs Stoichiometric Analysis of Crypthecodinium cohnii: A Guide for Microbial Metabolism Research
This article provides a comprehensive analysis of kinetic and stoichiometric modeling approaches for studying the metabolism of the heterotrophic marine dinoflagellate Crypthecodinium cohnii, a key organism for producing docosahexaenoic acid...
C1 Carbon Sources in Yeast: Comparing Platforms for Sustainable Biomanufacturing and Drug Development
This comprehensive review explores the critical challenge and opportunity of engineering yeast platforms to utilize C1 carbon sources—such as methanol, formate, and CO₂—for sustainable bioproduction.
FBA Strain Design Objective Functions: A Comprehensive 2024 Guide for Metabolic Engineers
This article provides a systematic comparison of Flux Balance Analysis (FBA) objective functions for microbial strain design, tailored for researchers and bioprocess developers.
Advanced Chinese Hamster Ovary (CHO) Cell Kinetic Modeling: A Comprehensive Guide to Validation, Application, and Optimization for Biopharmaceutical Development
This comprehensive article explores the critical process of validating kinetic models for Chinese Hamster Ovary (CHO) cells, the predominant host system for therapeutic protein production.
Unlocking Biofuel and Bioproduction: The Critical Link Between Central Carbon Metabolism and Lipid Accumulation in Yeast
This comprehensive review examines the intricate relationship between central carbon metabolism (CCM) and lipid biosynthesis in yeast, a cornerstone of metabolic engineering for biofuels, oleochemicals, and model disease studies.
Beyond the Cell: A Comprehensive Guide to Cell-Free Biocatalysis for Sustainable Alka(e)ne Production from Fatty Acids
This article provides a detailed examination of the cell-free biocatalytic conversion of fatty acids to alka(e)nes, a promising route for sustainable biofuel and biochemical synthesis.
Unlocking Genetic Resilience: A Comprehensive Guide to Cas12a CRISPR Screens for Multi-Gene Perturbation Tolerance
This article provides researchers and drug development professionals with a comprehensive framework for designing and executing Cas12a-based CRISPR screens to study cellular tolerance to multi-gene perturbations.
CarveMe vs ModelSEED vs RAVEN: A Comparative Guide to Genome-Scale Model Reconstruction for Metabolic Research
This article provides a comprehensive, comparative analysis of three leading software tools for genome-scale metabolic model (GEM) reconstruction: CarveMe, ModelSEED, and RAVEN Toolbox.